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成田 哲博

アクチンフィラメントは、細胞内で無数の重要な役割を果たしますが、その構造解析には、X線結晶解析やNMR法が使えません。

また、その直径は細く、ゆらぎも大きいため、電子顕微鏡法を用いても困難を伴います。そのため、アクチンフィラメントそのものやその制御メカニズムについては分かっていないことばかりです。

私は、電子顕微鏡写真を解析する新しい画像解析法の開発を通して、アクチンフィラメントのような繊維状タンパク質の動態と機能、および、制御タンパク質による制御メカニズムを探っています。

 

連絡先

narita.akihiro f.mbox.nagoya-u.ac.jp  (akihiroとfの間に@をいれてください)  内線6473

 

業績リスト

  1. Narita, A., Yasunaga, T., Ishikawa, T., Mayanagi, K. & Wakabayashi, T. (2001). Ca2+-induced switching of troponin and tropomyosin on actin filaments as revealed by electron cryo-microscopy. J.Mol.Biol. 308, 241-261.
  2. Narita, A., Takeda, S., Yamashita, A. & Maéda, Y. (2006). Structural basis of actin filament capping at the barbed-end:a cryo-electron microscopy study. EMBO J. 25, 5626-33.
  3. Imai, H., Narita, A., Schroer, T. A. & Maeda, Y. (2006). Two-dimensional averaged images of the dynactin complex revealed by single particle analysis. J Mol Biol 359, 833-9.
  4. Narita, A. & Maéda, Y. (2007). Molecular determination by electron microscopy of the actin filament end structure. J Mol Biol 365, 480-501.
  5. Popp, D., Yamamoto, A., Iwasa, M., Narita, A., Maeda, K. & Maeda, Y. (2007). Concerning the dynamic instability of actin homolog ParM. Biochem Biophys Res Commun 353, 109-14.
  6. Narita, A., Mizuno, N., Kikkawa, M. & Maeda, Y. (2007). Molecular determination by electron microscopy of the Dynein-microtubule complex structure. J Mol Biol 372, 1320-36.
  7. Mizuno, N., Narita, A., Kon, T., Sutoh, K. & Kikkawa, M. (2007). Three-dimensional structure of cytoplasmic dynein bound to microtubules. Proc Natl Acad Sci U S A 104, 20832-7.
  8. 成田 哲博 (2007) アクチンフィラメント端の伸長制御機構 月刊Bionics 3月号 314-317 オーム社
  9. Popp, D., Narita, A., Oda, T., Fujisawa, T., Matsuo, H., Nitanai, Y., Iwasa, M., Maeda, K., Onishi, H. & Maeda, Y. (2008). Molecular structure of the ParM polymer and the mechanism leading to its nucleotide-driven dynamic instability. Embo J 27, 570-9.
  10. Iwasa, M., Maeda, K., Narita, A., Maeda, Y. & Oda, T. (2008). Dual roles of Gln137 of actin revealed by recombinant human cardiac muscle alpha-actin mutants. J Biol Chem 283, 21045-53.
  11. Popp, D., Iwasa, M., Narita, A., Erickson, H. P. & Maeda, Y. (2009). FtsZ condensates: An in vitro electron microscopy study. Biopolymers.
  12. Oda, T., Iwasa, M., Aihara, T., Maeda, Y. & Narita, A. (2009). The nature of the globular- to fibrous-actin transition. Nature 457, 441-5.
  13. Popp, D., Iwasa, M., Maeda, K., Narita, A., Oda, T. & Maeda, Y. (2009). Protofilament formation of ParM mutants. J Mol Biol 388, 209-17. 
  14. 成田 哲博 (2009) 繊維状構造のための単粒子解析法  生物物理 49(6), 314-317
  15. Popp, D., Xu, W., Narita, A., Brzoska, A. J., Skurray, R. A., Firth, N., Goshdastider, U., Maeda, Y., Robinson, R. C. & Schumacher, M. (2010). Structure and filament dynamics of the pSK41 actin-like ParM protein: implications for plasmid DNA segregation. J Biol Chem.
  16. Popp, D., Narita, A., Iwasa, M., Maeda, Y. & Robinson, R. C. (2010). Molecular mechanism of bundle formation by the bacterial actin ParM. Biochem Biophys Res Commun 391, 1598-603.
  17. Popp, D., Iwasa, M., Erickson, H. P., Narita, A., Maeda, Y. & Robinson, R. C. (2010). Suprastructures and dynamic properties of mycobacterium tuberculosis FtsZ. J Biol Chem.
  18. Popp, D., Narita, A., Ghoshdastider, U., Maeda, K., Maeda, Y., Oda, T., Fujisawa, T., Onishi, H., Ito, K. & Robinson, R. C. (2010). Polymeric structures and dynamic properties of the bacterial actin AlfA. J Mol Biol 397, 1031-41.
  19. Takeda, S., Minakata, S., Koike, R., Kawahata, I., Narita, A., Kitazawa, M., Ota, M., Yamakuni, T., Maeda, Y. & Nitanai, Y. (2010). Two distinct mechanisms for actin capping protein regulation--steric and allosteric inhibition. PLoS Biol 8, e1000416.
  20. Popp, D., Narita, A., Maeda, K., Fujisawa, T., Ghoshdastider, U., Iwasa, M., Maeda, Y. & Robinson, R. C. (2010). Filament structure, organization, and dynamics in MreB sheets. J Biol Chem 285, 15858-65
  21. Ito, T., Hirayama, T., Taki, M., Iyoshi, S., Dai, S., Takeda, S., Kimura-Sakiyama, C., Oda, T., Yamamoto, Y., Maeda, Y. & Narita, A. (2011). Electron microscopic visualization of the filament binding mode of actin-binding proteins. J Mol Biol 408, 26-39.
  22. Ito, T., Narita, A., Hirayama, T., Taki, M., Iyoshi, S., Yamamoto, Y., Maeda, Y. & Oda, T. (2011). Human spire interacts with the barbed end of the actin filament. J Mol Biol 408, 18-25.
  23. Narita, A., Oda, T. & Maeda, Y. (2011). Structural basis for the slow dynamics of the actin filament pointed end. Embo J 30, 1230-7.
  24. Narita, A. (2011). Merits of the double-stranded form of the actin filament revealed by structures of the filament ends. Communicative & Integrative Biology 4, 692-695
  25. Narita, A. (2011). Minimum requirements for the actin-like treadmilling motor system. BioArchitecture 1, 205-208
  26. Narita, A., Mueller, J., Urban, E., Vinzenz, M., Small, J. V. & Maeda, Y. (2012). Direct Determination of Actin Polarity in the Cell. J. Mol. Biol. 22 359-368
  27. Vinzenz, M., Nemethova, M., Schur, F., Mueller, J., Narita, A., Urban, E., Winkler, C., Schmeiser, C., Koestler, S. A., Rottner, K., Resch, G. P., Maeda, Y. & Small, J. V. (2012). Actin branching in the initiation and maintenance of lamellipodia. J Cell Sci.  J Cell Sci 125, 2775-85.
  28. *Popp, D., *Narita, A., Lee, L. J., Goshdastider, U., Xue, B., Srinivasan, R., Balasubramanian, M. K., Tanaka, T. & Robinson, R. C. (*: equally contributing authors) (2012). A novel actin-like filament structure from Clostridium tetani. J Biol Chem. 287, 21121-9.
  29. *Popp, D., *Narita, A., Lee, L. J., Larsson, M. & Robinson, R. C. (2012). (*: equally contributing authors) Microtubule-like Properties of the Bacterial Actin Homolog ParM-R1. J Biol Chem 287, 37078-88.
  30. Iwasa, M., Aihara, T., Maeda, K., Narita, A., Maeda, Y. & Oda, T. (2012). Role of the actin ala-108-pro-112 loop in actin polymerization and ATPase activities. J Biol Chem 287, 43270-6.
  31. 成田 哲博 (2013). 電子線トモグラフィーによる細胞内アクチンフィラメント構造解析 顕微鏡 48, 78-83
  32. Mueller, J, Pfanzelter, J, Winkler, C, Narita, A, Clainche, CL, Nemethova, M, Carlier, M, Maeda, Y, Welch, MD, Ohkawa, T, Schmeiser, C, Resch, GP, Small, JV (2014).   Electron Tomography and Simulation of Baculovirus Actin Comet Tails Support a Tethered Filament Model of Pathogen Propulsion, PLOS Biol., doi/10.1371/journal.pbio.1001765
  33. Hiroshi Imai, Akihiro Narita, Yuichiro Maéda, Trina A. Schroer (2014), Dynactin 3D Structure: Implications for Assembly and Dynein Binding, J. Mol. Biol. 426(19), 3262-3271
  34. Y. Shigemitsu, N. Iwaya, N. Goda, M. Matsuzaki, T. Tenno, A. Narita et al. (2016). Nuclear magnetic resonance evidence for the dimer formation of beta amyloid peptide 1-42 in 1,1,1,3,3,3-hexafluoro-2-propanol. Anal Biochem 498, 59.
  35. #Shimin Jiang, #Akihiro Narita, #David Popp, #Umesh Ghoshdastider, Lin Jie Lee, Ramanujam Srinivasan, Mohan K. Balasubramanian, Toshiro Oda, Fujiet Koh, Mårten Larsson, Robert C. Robinson (2016), A novel plasmid-segregating actin from Bacillus thuringiensis forms dynamic antiparallel filaments and tubules, PNAS 113: E1200-1205  (#: Equally contributing authors)All authors contributed equally to this work

  36. A. Narita, E. Usukura, A. Yagi, K. Tateyama, S. Akizuki, M. Kikumoto et al. (2016). Direct observation of the actin filament by tip-scan atomic force microscopy. Microscopy (Oxf) 65, 370.
  37. E. Usukura, A. Narita, A. Yagi, S. Ito, J. Usukura. (2016). An Unroofing Method to Observe the Cytoskeleton Directly at Molecular Resolution Using Atomic Force Microscopy. Scientific reports 6, 27472. All authors contributed equally to this work
  38. Masaki Makihara, Takashi Watanabe, Eiji Usukura, Kozo Kaibuchi, Akihiro Narita, Nobuo Tanaka and Jiro Usukura (2016), A new approach for the direct visualization of the membrane cytoskeleton in cryo-electron microscopy: a comparative study with freeze-etching electron microscopy. Microscopy 65, 488-498
  39. Jiang S, Ghoshdastider U, Narita A, Popp D, Robinson RC. Structural complexity of filaments formed from the actin and tubulin folds. Communicative & integrative biology. 2016;9:e1242538.

プレス発表

“細胞が形状を変えながら移動する謎の一端を解明 - アクチンフィラメント端での伸縮制御メカニズムが明らかに –“

2006年 11/17 科学技術振興機構, 理化学研究所, 名古屋大学

日本経済新聞、日経産業新聞、日刊工業新聞、中日新聞、科学新聞の5紙に掲載

 

受賞歴

風戸研究奨励賞(2007)

文部科学大臣表彰若手科学賞(2008)

 

学会活動

1998-1999 生物物理若手の会会長

2009-2010 日本顕微鏡学会第66回学術講演会実行委員

2011-2012 日本生物物理学会第50回年会実行委員

2012-2013 日本顕微鏡学会第57回シンポジウム実行委員

2014-2015 日本顕微鏡学会第71回学術講演会プログラム委員

2015-     日本顕微鏡学会理事

 

 

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